DNA Extraction and Purification
Agilent Technologies High Sensitivity D1000 Reagents
For the high sensitivity analysis of DNA from 35 to 1000 bp. Includes ladder and sample buffer.
Zymo Research Corporation ZymoPURE II™ Plasmid Maxiprep (10 preps)
The ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 g/l), EndoZero, vaccine-grade, and transfection-ready. The ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Agilent Technologies D1000 ScreenTape for DNA analysis
For the analysis of DNA from 35 to 1000 bp. Includes 7 ScreenTape devices.
Edge Biosystems Inc Performa® DTR Gel Filtration Cartridges 108 pack
The gold standard in gel filtration based dye terminator removal!Performa® DTR utilizes a fully hydrated gel filtration matrix that provides for a convenient and simple method of dye terminator removal for sequencing cleanup. Performa DTR ensures removal of BigDye® Terminator v3.1 or other dye terminators, along with dNTPs, salts and other low molecular weight materials from sequencing reactions. DNA primers and fragments up to 20 bases, buffers, nucleotides labeled with biotin, isotopes and other assorted markers are also eliminated. To minimize the potential for interference with sequencing applications, no preservatives, salts, or buffers are used in the preparation of Performa DTR.
Zymo Research Corporation ZymoPURE II™ Plasmid Gigaprep (5 preps)
The ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry, which enables the highest yields and concentration of plasmidDNA (up to 3 g/l) directly from a spin-column. Coupling ZymoPURE with the innovative EndoZero™ Spin-Columns, to eliminate endotoxins, achieves EndoZeroplasmid DNA ( 0.025 EU/g of plasmid DNA), making it suitable for transfection, restriction endonuclease digestion, in vivo studies, bacterial transformation, PCRamplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ II Plasmid Gigaprep Kit contains colored buffers that permit error-free visualization and identification of completebacterial cell lysis and neutralization. Also, bottle top filters are included for rapid clearing of the lysate.
LGC GENOMICS LLC (Lucigen) QuickExtract DNA Extraction Solution 1.0, 50 mL
The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.
Covaris Inc g-TUBE for DNA Shearing, 10/pk
10 individually-wrapped g-TUBES for shearing DNa 6kb to 20 kb and one g-TUBE Prep Statio
Agilent Technologies DNA ScreenTape Analysis, 1 each
D1000 Reagents. For the analysis of DNA from 35 to 1000 bp. Includes ladder and sample buffer sufficient for 112 samples.
![Zymo Research Corporation ZymoBIOMICS DNA Miniprep Kit (50 preps)Includes: [D4304] ZymoBIOMICS Miniprep Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1[D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/OS-D4300ZYMOBIOMICS-DNA-MINIPREP-KIT.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS DNA Miniprep Kit (50 preps)Includes: [D4304] ZymoBIOMICS Miniprep Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1[D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Zymo Research Corporation ZR BashingBead Lysis Tubes (0.1 & 0.5 mm), 50 tubes
The state of the art, ultra-high density beads are fracture resistant, chemically inert, and ideal for disrupting tough-to-lyse biological samples. These beads are ideal for tough-to-lyse bacteria, yeast, fungi and algae.
New England Biolabs, Inc. Thermolabile Proteinase K – 30 units
Thermolabile Proteinase K (TLPK) is an engineered, subtilisin-related serine protease that will hydrolyze a broad variety of peptide bonds. It preferentially cleaves the peptide bond at the carboxyl side of aliphatic or aromatic amino acid residues. TLPK can be completely inactivated by incubation at 55°C for 10 minutes, which allows for subsequent enzymatic steps in the same reaction vessel.
Macherey-Nagel NucleoSpin Tissue, 250 preps
NucleoSpin Tissue (250) 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K
LGC GENOMICS LLC (Lucigen) QuickExtract DNA Extraction Solution 1.0, 5 mL
The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.
Zymo Research Corporation Zymoclean™ Gel DNA Recovery Kit (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)
Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing your DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes Fast-Spin column technology to yield high-quality DNA in just 15 minutes. DNA purified using the Zymoclean™ Gel DNA Recovery Kit is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
Zymo Research Corporation ZymoBIOMICS™ Microbial Community DNA Standard (2000 ng)
One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem.